Directional Keys Inc.
Published eBooks Currently Available for Purchase on Amazon
Peer-Reviewed Scientific-Research Papers Published
Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss
Abstract
Bone is a dynamic tissue, which continually adapting its structure during the development process. The aim of the study was to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization using MG-63 cells.
The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lorraine Marie Hachfeld and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with a safe and nontoxic profile on MG-63 cell line. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10μg/mL, respectively in the UT-DMEM+BT-TI, while 314.3% at 10μg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10μg/mL, respectively. Collagen was significantly increased by 180% and 128.1% at 0.1 and 1μg/mL, respectively in the UT-DMEM+BT-TI group, while 84.4% and 69.7% at 1 and 10μg/ mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Furthermore, BT-DMEM+BT-TI group showed a significant increased collagen level by 123.4% and 184.8% at 1 and 10 μg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 385.1% at 0.1μg/mL in the UT-DMEM+BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10μg/ mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10μg/mL, respectively as compared with the untreated group.
The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. It regulates the osteoblast function, improves the bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
Keywords: Biofield healing; Bone mass; Osteosarcoma cells; Calcium absorption; Vitamin D; Bone mineralization
Abbrevations: CAM: Complementary and Alternative Medicine; NCCAM: National Center for Complementary and Alternative Medicine; MG-63: Human Bone Osteosarcoma Cells, ALP: Alkaline Phosphatase; DMEM: Dulbecco’s Modified Eagle’s Medium; FBS: Fetal Bovine Serum; FBS: Fetal Bovine Serum; EDTA: Ethylenediaminetetraacetic Acid; UT: Untreated, BT: Biofield Energy Treated, TI: Test Item
Conclusion
Biofield Energy Treated vitamin D3 and DMEM was studied for bone health parameters. MTT study for cell viability data showed that a significant improved cell viability with more than 79% among all the tested groups. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10μg/mL, respectively in the UT-DMEM+BT-TI, while 314.3% at 10μg/mL in the BT- DMEM+BT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10μg/mL, respectively. The level of collagen was significantly increased by 180%, 128.1%, and 38.6% at 0.1, 1, and 10μg/mL, respectively in the UT-DMEM+BT-TI, while 84.4% and 69.7% at 1 and 10μg/mL, respectively in the BT- DMEM+UT-TI group. In addition, collagen level was increased by 44.4%, 123.4%, and 184.8% at 0.1, 1, and 10μg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone mineralization percent was significantly increased by 385.1%, 28.7%, and 19.7% at 0.1, 1, and 10μg/mL, respectively in the UT-DMEM+BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10μg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10μg/mL, respectively as compared with the untreated group.
Thus, experimental results demonstrated that Biofield Energy Healing Treatment can be utilized in managing various bone health parameters viz. collagen, bone mineralization, and ALP to combat the bone disorders. Thus, The Trivedi Effect®-Consciousness Energy Healing based vitamin D3 showed a significant improved bone health, which supports its use as the best nutritional supplement to treat bone related disorders such as rickets, deformed bones, osteomalacia, osteoma, osteoporosis, Paget’s disease, aging, bone and/or joint pain, increased tendency of fractures, hormonal imbalance, stress, and other associated bone diseases. Besides, this approach can also supports its use against many various immune related disease conditions and worked as anti-arthritic, anti- aging, anti-apoptotic, anti-osteoporosis, anti-inflammatory, anti- stress, anti-cancer, wound healing, and anti-fibrotic roles. It would improve the cell-to-cell communication, cell differentiation, cell cycling and proliferation, normal cell growth, neurotransmission, skin health, and also in vital cardiovascular functions. Besides, it can also be utilized against Dermatitis, Multiple Sclerosis, Pernicious Anemia, Asthma, Irritable Bowel Syndrome, Diabetes, Diverticulitis, Hashimoto Thyroiditis, Myasthenia Gravis, Sjogren Syndrome, Aplastic Anemia, Hepatitis, Parkinson’s disease, Atherosclerosis, and many more.
Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss Using MG-63 Cells.
Published July 17, 2018,
Orthopedic Research Online Journal,
Volume 3 (Issue 5), pp. 286-292
OPROJ.000572.2018
DOI: 10.31031/OPROJ.2018.03.000572
ISSN: 2576-8875
Copyright © 2018 All rights are reserved by Lorraine Marie Hachfeld
Impact of Biofield Energy Treated Herbomineral Formulation (The Trivedi Effect®) on Mouse
Abstract:
The use of herbomineral formulation in the healthcare sector for different chronic diseases is gaining popularity due to its fewer side effects, high safety profile, and cost effectiveness. A new proprietary herbomineral formulation was formulated, consisting of four essential ingredients viz. herbal root extract (ashwagandha), and minerals (zinc, magnesium, and selenium).
The study aims to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine dendritic (DCs) and splenocyte cells. The herbomineral formulation was divided into two parts; one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The DCs were treated with the Biofield Energy Treated test formulation at different concentrations (i.e. 1.05 to 1052.5 µg/mL) for 24 hours, and the results showed significant (p≤0.001) suppression of TNF-α levels at all the tested concentrations with a maximum percentage decrease by 43.64% at 5.2 µg/mL concentration in the Biofield Treated formulation as compared with the untreated test formulation. Further, the Biofield Treated formulation also demonstrated inhibition of MIP-1α and IL-1β at a concentration range of 0.0000105 to 10.5 µg/mL in LPS stimulated splenocyte cells. There was a significant (p≤0.001) inhibition of MIP-1α (26.52%) and IL-1β (35.28%) in the Biofield Treated test formulation at concentration 0.000105 µg/mL and 0.0000105 µg/mL, respectively in comparison to the untreated test formulation.
Overall, these results suggest that the Biofield Energy Healing based herbomineral formulation (The Trivedi Effect®) significantly down-regulated the tested cytokines expression in DCs and splenocyte cells as compared to the untreated formulation.
Therefore, the Biofield Healing based formulation might be useful as a better anti-inflammatory product for many chronic and acute inflammatory disease conditions and autoimmune disorders.
The Biofield Energy Treatment based formulation can also be effectively applied in cases of organ transplants, stress management and anti-aging by improving overall health.
Impact of Biofield Energy Treated Herbomineral Formulation (The Trivedi Effect®) on Mouse Dendritic and Splenocyte Cells for Modulation of Pro-inflammatory Cytokines,
International Journal of Immunology.
Vol. 4, No. 5, 2016, pp. 35-45.
DOI: 10.11648/j.iji.20160405.12
Copyright © 2016 Authors retain the copyright of this article.
Evaluation of Immunomodulatory in Female Rats after Oral Administration of the Treated Formulation
Evaluation of Immunomodulatory Parameters in Female Sprague Dawley Rats after Oral Administration of the Biofield Energy Healing Treated Herbomineral Formulation,
American Journal of BioScience.
Vol. 5, No. 6, 2017, pp. 104-113.
DOI: 10.11648/j.ajbio.20170506.12
Copyright © 2017 Authors retain the copyright of this article.
Assessment of Counsciousness Energy Treatment after Oral Aministration of Formulation in Male Rats
Assessment of Consciousness Energy Healing Treatment on Immune Biomarkers After Oral Administration of Herbomineral Formulation in Sprague Dawley Male Rats,
Biochemistry and Molecular Biology.
Vol. 2, No. 6, 2017, pp. 110-119.
doi: 10.11648/j.bmb.20170206.17
Copyright © 2017 Authors retain the copyright of this article.
Impact of Biofield Energy Treated Formulation on the Immune Biomarkers and Blood of Female Rats
Impact of Biofield Energy Treated (The Trivedi Effect®) Herbomineral Formulation on the Immune Biomarkers and Blood Related Parameters of Female Sprague Dawley Rats, American Journal of Life Sciences.
Vol. 5, No. 6, 2017, pp. 150-159.
doi: 10.11648/j.ajls.20170506.11
Copyright © 2017 Authors retain the copyright of this article.
An Investigation of Energy Treatment to Modulate the Immunomodulatory Effect of Formulation in Rats
An Investigation of The Trivedi Effect®-Energy of Consciousness Healing Treatment to Modulate the Immunomodulatory Effect of Herbomineral Formulation in Male Sprague Dawley Rats,
American Journal of Biomedical and Life Sciences. Vol. 5, No. 6, 2017, pp. 144-153.
doi: 10.11648/j.ajbls.20170506.16
Copyright © 2017 Authors retain the copyright of this article.
More Peer-reviewed Scientific-Research Papers Published
Investigation of Sodium Selenate Treated with the Energy of Consciousness (Trivedi Effect®)
Excerpt from Abstract:
Sodium selenate is used for the prevention and treatment of various disorders like cancer, diabetes, inflammation diseases, etc.
The objective of the current study was to evaluate the effect of The Trivedi Effect®-Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physicochemical, spectral, and thermal properties of The Trivedi Effect®-Biofield Energy Treated sodium selenate. Sodium selenate was divided into two parts – one part was control without any treatment and another part was treated with The Trivedi Effect®-Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect®-Energy of Consciousness Healing Treated sample.
The PXRD analysis showed the significant alteration of the crystallite size of the treated sample in the range of -36.36% to 133.24% as compared to the control sample. However, the average crystallite size of the treated sample was significantly decreased by 8.31% as compared to the control sample. Overall, the crystal morphology of the treated sample was different from the control sample. The particle sizes d10, d50, and d90 values of the treated sample were significantly decreased by 3.40%, 9.75%, and 8.52%, respectively as compared to the control sample. Consequently, the surface area of the treated sample was significantly increased by 5.26% from the control sample. Both the control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 887 cm-1 due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of both the control and treated samples were at 204.1 and 204.0 nm, respectively. TGA analysis revealed that the total weight loss of the treated sample was reduced by 1.24% as compared with the control sample. The DSC analysis showed that the treated sample (588.77°C) had higher melting point than the control sample (588.60°C). The latent heat of fusion was reduced in the treated sample by 8.01% compared to the control sample.
Thus, The Trivedi Effect®-Biofield Energy Healing might lead to generate a new polymorphic (enantiotropic) form of sodium selenate which would be more soluble, bioavailable, and thermally stable compared with the untreated sample.
The Trivedi Effect treated sodium selenate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, stress, aging, infectious diseases, cancer, diabetes, heart diseases, Alzheimer’s disease, etc.
Investigation of Physicochemical, Spectral, and Thermal Properties of Sodium Selenate Treated with the Energy of Consciousness (The Trivedi Effect®),
American Journal of Life Sciences.
Vol. 5, No. 1, 2017, pp. 27-37.
doi: 10.11648/j.ajls.20170501.15
Copyright © 2017 Authors retain the copyright of this article.
Evaluation of Impact of Biofield Energy Healing Treatment of Magnesium Gluconate
Evaluation of the Impact of Biofield Energy Healing Treatment (The Trivedi Effect®) on the Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate.
International Journal of Nutrition and Food Sciences. Vol. 6, No. 2, 2017, pp. 71-82.
DOI: 10.11648/j.ijnfs.20170602.13
Copyright © 2017 Authors retain the copyright of this article.
A Comprehensive Analytical Evaluation of Trivedi Effect® on Zinc Chloride
A Comprehensive Analytical Evaluation of the Trivedi Effect® - Energy of Consciousness Healing Treatment on the Physical, Structural, and Thermal Properties of Zinc Chloride,
American Journal of Applied Chemistry.
Vol. 5, No. 1, 2017, pp. 7-18.
DOI: 10.11648/j.ajac.20170501.12
Copyright © 2017 Authors retain the copyright of this article.
Structural and Natural Isotopic Abundance Analysis of Magnesium Gluconate
Structural and Natural Isotopic Abundance Analysis of Magnesium Gluconate Treated with Energy of Consciousness (The Trivedi Effect®) Using LC-MS and NMR,
Chemical and Biomolecular Engineering.
Vol. 2, No. 3, 2017, pp. 124-134.
doi: 10.11648/j.cbe.20170203.11
Copyright © 2017 Authors retain the copyright of this article.
Spectroscopic Analysis of Ashwagandha Root Extract after Treatment
LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®),
European Journal of Biophysics.
Vol. 5, No. 2, 2017, pp. 27-37.
DOI: 10.11648/j.ejb.20170502.11
Copyright © 2017 Authors retain the copyright of this article.
Impact of Consciousness Energy Healing Treatment on Ashwagandha Root Extract
Impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on Physical, Spectroscopic, and Thermal Properties of Withania somnifera (Ashwagandha) Root Extract,
International Journal of Food Science and Biotechnology. Vol. 2, No. 1, 2017, pp. 6-15.
doi: 10.11648/j.ijfsb.20170201.12
Copyright © 2017 Authors retain the copyright of this article.
NEW! Top-Level Master Healing energy.
The Immune Support packages have been up-leveled with this new higher level of healing energy at the previous price for a limited time.